加入WTO后我国数字农业的发展

基于我国数字农业现状的基础上，分析了数字农业对我国农业发展的影响，提出了我国数字农业发展的研究策略。     

Construction of mouse ESC line stably expressing Pdx1 by Tet-On system

AIM To construct the mouse embryonic stem cell （ESC） line with stable pancreatic and duodenal homeobox 1 （Pdx1） expression by Tet-On system, which may lay a foundation for further research on the differentiation of Pdx1⁺ definitive endoderm cells into pancreatic cells. METHODS The Pdx1-overexpressing lentiviral vector with green fluorescent protein marker and puromycin resistance was constructed by Tet-On system and was used to infect the mouse ESC. The cells were divided into 3 groups： blank control group （ESC group）, empty lentivirus control group （PDX1^- ESC group） and Pdx1 lentivirus transfection group （PDX1⁺ ESC group）. Flow cytometry was used to detect the transfected cells after screening by doxycycline （DOX）. The function of Tet-On system and the expression of Pdx1 gene were detected. The transfected cells in PDX1^- ESC group and PDX1⁺ ESC group were sorted by flow cytometry, and constructed ESC line with stable expression of Pdx1 and negative control ESC line were verified. RESULTS （1） The positive rates of transfected cells in PDX1^- ESC group and PDX1⁺ ESC group were 90.72% and 94.01% after screening by DOX, respectively. The positive rates of transfected cells in PDX1^- ESC group and PDX1⁺ ESC group was 97.84% and 98.13% after sorting by flow cytometry, respectively. （2） With DOX, green fluorescence was observed in PDX1^- ESC group and PDX1⁺ ESC group. The mRNA and protein expression of Pdx1 was significantly increased in PDX1⁺ ESC group （P<0.05）. Without DOX, no green fluorescence was observed in the cells of the 3 groups, and no significant difference in the mRNA and protein expression of Pdx1 was observed （P>0.05）. （3） After 3 months of cryopreservation, the cell lines still survived in resuscitation culture and were regulated by DOX. CONCLUSION Using Tet-On system, the mouse ESC line with inducible Pdx1 expression were successfully established and could be used as an effective cell model to research the differentiation of Pdx1⁺ definitive endoderm cells into pancreatic cells.     

水果黄瓜新品种'青玉'的选育

水果黄瓜新品种'青玉'是以自交系HQ6548为母本,自交系GY4237为父本经杂交而成的一代杂种.该品种植株长势强,早熟,叶片中等,节间稳定,茎粗壮,连续结瓜能力强;瓜长15～17 cm,横径约3 cm,瓜条顺直,口感清脆,瓜色浅绿色,刺瘤稀疏,单瓜质量130～170 g,产量高;抗霜霉病、白粉病和黄瓜花叶病毒病.适宜在山东、河北、河南、海南等四季保护地栽培及山东夏季露天栽培.     

春大葱接秋萝卜套栽越冬甘蓝三种三收高效栽培技术

介绍了春大葱、秋萝卜套种越冬甘蓝的模式,该模式一年三种三收,年内土地利用时空上基本无缝对接,实现了作物之间的轮作倒茬,有利于土壤改良和合理利用,拉高复种指数.详细阐述了各个茬次的栽培技术要点,生产过程省工、省时,便于机械化作业,人工投入比较少,经济效益和社会效益十分显著.     

LAS对土壤微生物生物学指标的影响及降解条件的正交实验分析

用正交实验方法研究了徐州地区4种土壤在不同组合条件下土壤微生物数量、酶活性及LAS的降解条件。结果表明,LAS对土壤细菌生长有促进作用,对真菌则有抑制作用,而对放线菌无明显影响。LAS对转氨酶和脱氢酶均有抑制作用。微生物数量波动最大的组合条件为荒土、30℃、LAS浓度50mg·kg-1、时间8d;LAS最大降解的组合条件为河土、25℃、LAS浓度20mg·kg-1、时间8d(降解率为33.2%);土壤酶活性减少最多的组合条件为麦田土、25℃、LAS浓度30mg·kg-1、时间8d(转氨酶活性减少0.0594,脱氢酶抑制率达49.58%)。另外,土壤对LAS污染的环境效应与土壤类型和性质等因素有关。     

Kisspeptin-10对蛋鹌鹑卵巢发育与内分泌的影响

为研究Kisspeptin-10(Kp-10)对禽类生殖内分泌的影响,将75羽22日龄雌鹌鹑随机分为3组,分别腹腔注射300μL生理盐水(对照组),注射0.1 nmol Kp-10(低剂量组)和1 nmol Kp-10(高剂量组),每日注射1次,连续注射3周。记录鹌鹑开产情况,并于60日龄时采集血液和卵泡组织,测定生殖激素与受体的mRNA表达。结果:与对照组相比,注射Kp-10组鹌鹑产蛋率显著提高,且大卵泡数显著增加,血液中雌激素水平显著升高;低剂量组输卵管重显著增加,但体重、肝脏重和小卵泡重无显著变化。Real-time PCR结果显示,Kp-10处理显著下调小卵泡及F1级卵泡上促黄体激素受体(LHR)、促卵泡激素受体(FSHR)和促性腺激素释放激素Ⅰ(GnRH-Ⅰ)的基因表达。     

马尾松不同抚育方式对林地生产的影响

对马尾松幼林采用全面劈草、全面劈草局部松土、全面除草松土3种抚育方式对林地植被生长、表层土壤变化及幼林生长的影响进行研究,结果表明:植被覆盖率、植被枯落物随抚育强度提高而降低,而土壤侵蚀则随抚育强度提高而增强,马尾松幼林生长与抚育强度提高成正比.在南方马尾松集约经营的山区,宜提倡带状抚育,既可促进幼林生长,又能维护地力.     

多媒体教学环境下大学生思想政治教育研究

在全球化浪潮的背景下,我国的社会主义意识形态建设面临巨大挑战,信息领域的最大受众——大学生们最先受到西方敌对势力意识渗透的影响。分析多媒体技术环境下思想政治教育工作的的途径,以促进大学思想政治教育的持续开展。     

东南石栎轻基质网袋容器育苗试验

运用正交设计,采用不同的基质配比、网袋规格、缓释肥数量对东南石栎(Lithocarpus harlandii)进行轻基质网袋容器育苗试验,结果表明,不同网袋规格、基质配比对东南石栎容器苗的粗生长和高生长都具有显著的影响,不同的缓释肥数量对苗木的粗生长和高生长都没有显著影响,东南石栎轻基质网袋容器育苗采用5cm×(10-12)cm的无纺布网袋规格,东北泥炭与砻糠体积比7:3为基质,适量加施缓释肥(2.5 kg/m3),其1年生苗高可达27.23 cm,地径0.32 cm.     

Expression and clinical significance of RhoC and Ki-67 in human esophageal squamous cell carcinoma tissues

AIM: To investigate the expression and significance of RhoC and Ki-67 in human esophageal squamous cell carcinoma (ESCC) tissues.METHODS: The expression of RhoC and Ki-67 was detected in 52 specimens of ESCC by the method of immunohistochemistry. The clinicopathological features were also analyzed.RESULTS: The expression of RhoC was detected in 32 of the total 52 (61.5%) cases of human ESCC tissues, significantly higher than that in the adjacent histologically normal epithelium, which was only in 11 of 37 cases (29.7%, P<0.05). RhoC expression was closely correlated with clinical tumor-node-metastasis (TNM) stage (P<0.05) and lymph node metastasis (P<0.05) in ESCC. The over-expression of RhoC was positively correlated with Ki-67 in ESCC (r=0.322, P<0.05).CONCLUSION: The over-expression of RhoC protein significantly correlates with advanced TNM stage, lymph node metastasis and cell proliferation ability of ESCC. Therefore, RhoC may be a new auxiliary parameter for early diagnosis and prognostic evaluation of ESCC.